Take oocyte and sperm out individually and fertilize in vitro, after embryo culture and transfer into uterus.

Take oocyte and sperm out individually and fertilize in vitro, beginning the fertilization, embryo culture in tube or culture plate. After develop to embryo, it will transfer to uterus.

According to the menstrual cycle to stimulate ovulation. It will use ovulate stimulate injections and track the growth of follicles by ultrasound and hormone testing. When the follicle mature, hCG inject and do the oocyte retrieval after 34-36 hours inject.

Fertilize after several hours of oocyte retrieval, which depends on the maturity of oocyte. Culture in the incubator and check the fertilization condition next day. Keep culture to 4-8 cell stage or blastocyst stage of embryo and transfer into the uterus. Extra embryos can freeze and store for other day to use.